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Hi, I have been using Flowjo to analyze my flow data. When I plot Fsc vs ssc, it turns out most of the cell events are on the edges? Is there I can do to fix it to make all the events in frame?
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I isolate PBMCs from leukocyte reduction filters derived from healthy donors. The filters are purchased from Rhode Island Blood Center. I use HBSS buffer with 2.5% surcose and 5mM EDTA (PH=7.2) to...
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