Rucsanda Pinteac

3 Questions 6 Answers 0 Followers

Questions related from Rucsanda Pinteac

How can I detect cleaved-caspase 3 by western blotting?

Hi everyone, I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6...

09 September 2019 10,015 4 View

Does anyone use L-Glutamic Acid from Sigma (REF: G5667)?

Hi everyone, We want to perform an excitotoxicity assay on primary cortical mouse neurons and we want to use this L-Glutamic Acid. If anyone has used this product, how you dilute the glutamate?...

04 April 2018 8,637 4 View

Which is the best protocol to transfect astrocytes (primary cell culture) with Lipofectamine 3000?

Hi everyone, We are trying to transfect astrocytes (24 wells plate) and we followed the original protocol with the high concentration for lipofectamine 3000 (1,5 uL). We had a high amount of...

03 March 2018 9,379 10 View