Ruchi Jain

3 Questions 2 Answers 0 Followers

Questions related from Ruchi Jain

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

21 December 2020 1,554 4 View

What are acceptable enrichment in Phage ELISA?

I generated a phage library against a specific peptide. After several rounds of panning, I do see an enrichment in specific phages but only 2 fold as compared to the BSA control. What is the best...

09 November 2020 4,890 1 View

Mouse scFv library construction?

Hi, I am having issues with the overlap PCR in making mouse scFv library. When I get the right size band, then post cloning, I am getting a shorter fragment inserted in the plasmid. All my...

21 May 2020 9,859 0 View