Rubha Devi

3 Questions 8 Answers 0 Followers

Questions related from Rubha Devi

Why need of more lentivirus to transduce the NPCs?

Hi All, I am doing the lenti-transduction of viral constructs in Neural progenitor cells, I require at least 2 wells of one six well plate lentivirus prepared from 293T to infect the one 12 well...

12 June 2024 9,553 1 View

Why my input (cell lysate)doesn't show clear signal as bound protein?

Hi. I ran the western blot of my immunoprecipitated sample. I don't know why my protein band appears like a smear? what could be the possible reason? I loaded 3% of input that is cell lysate and...

14 August 2023 1,797 9 View

I unable to get clone for mouse protease gene (1700bp) into pET30b System- can anyone help?

I am trying to ligate my insert (1700bp) into pET30b vector (5.4kb) with Nde1 and Xho1 restriction site in order to use N terminal histag purification. The 1: 3 ratio of vector (50ng) to insert...

21 May 2015 3,439 4 View