Does anyone have a protocol for staining late stage drosophila embryos using phalloidin?

I am trying to stain stage 17 embryos using phalloidin without any success. I am fixing the embryos in a 1:1 mixture of heptane/4% PFA after dechorionation in 50% bleach. I devitellinize by hand...

30 January 2022 2,258 1 View