Ruairidh Edwards

2 Questions 2 Answers 0 Followers

Questions related from Ruairidh Edwards

His-tagged protein not binding to nickel beads, any help?

I am trying to purify a fusion protein using the E.Coli expression system. I have successfully expressed my protein and verified this using an anti-His antibody and an antibody that recognises my...

05 February 2017 4,341 5 View

How can only a long isoform of a protein be expressed and not the short?

I might be over thinking this but my problem lies with understanding how only a long protein isoform can be expressed when the short isoform is encoded by the same region, just 85 amino acids...

12 December 2014 3,751 4 View