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Questions related from Roy Mathew Francis
It seems like standard GWAS is not suitable for case/control SNP association testing in closely related individuals. What are the alternatives?
29 January 2018 1,750 2 View
Is it possible to accurately and reliably estimate amplicon length or amplicon length distribution from qPCR melting curve/dissociation curve? Are there any articles on this? If it matters, my...
02 March 2017 2,792 3 View
Epigenetic marks such as DNA methylation and histone modifications are maintained during DNA replication and cell division*. What happens to epigenetic marks during a PCR...
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I am extracting RNA from Zebrafish tissue (Brain, Testes, Embryos etc) using standard Trizol total RNA extraction protocol. The RNA concentration and yeild are good. Checked on Qubit. RNA quality...
02 December 2015 7,748 4 View
I am using the standard Trizol protocol to extract total RNA from Zebrafish brain and testes. I would like to get an idea of how much RNA yield to expect. If you have used a different...
16 September 2015 9,805 0 View
Nucleic acids (DNA and RNA)/nucleus often needs to be stained to identify cell types and cellular location for use in confocal imaging, LCM (laser capture microdissection), flow cytometry etc....
23 April 2014 3,421 4 View
Some organisms can have far too many alleles (for ex. Na > 18) at some or many loci. This high variability leads to higher variation between individuals within a population than between...
17 January 2014 7,089 17 View
