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Questions related from Roxana Nezami
Hi everyone, I have a question in the case of, codons and regions in the whole DNA. I wanted to design a pair of primers that include 167, 198 and 200 codons. But I couldn't find them in the whole...
30 November 2022 3,559 1 View
I have done a lot of PCR to identify a mutation in beta-tubulin. I have two sets of primers, one is for mutant and one is for wild type. I did nearly 100 PCR but I have a problem getting a good...
28 November 2022 5,811 6 View
I'm doing a PCR for detection of Mutation on position 167 in beta tubulin. It is a Nested PCR which a second reaction was performed using the primers Fs + R2 (141 bp) and F1 + Rr (212 bp). The Fs...
19 June 2022 5,741 3 View
Hi everyone I have made a Stock Solution of my primer 100 uM, and I also have a Working Solution of 10 uM. can I convert my working solution 10uM to 5uM directly?! if I want to make a 5uM working...
21 January 2022 5,547 4 View
Hi everyone I tried to do PCR, and I expected to get a band in 121, but is my third PCR that I have gotten 400bp band. I Checked my primer and all of the other parameters. it should be mentioned...
09 December 2021 2,154 10 View
Hello everyone, I have all the hookworm ITS-2 sequences and I want to check where the primers are present and the size of the amplicon with an alignment software (NCBI Blast). what should I do? I...
16 October 2021 1,573 6 View
i want to confirm my finding regarding Ancylostoma Caninum eggs by molecular studies, which is ``ITS-2 amplification``. but the number of eggs in my samples is not to much and i cant get any band...
30 August 2021 1,030 11 View
Does anybody know what these can be? These are my finding after fecal flotation for dogs and I'm wondering if anybody can have an idea about these two photos? Thank you in advance
06 June 2021 9,973 0 View
