7 Questions 23 Answers 0 Followers
Questions related from Roxana Nezami
Hi everyone, I have a question in the case of, codons and regions in the whole DNA. I wanted to design a pair of primers that include 167, 198 and 200 codons. But I couldn't find them in the whole...
30 November 2022 3,497 1 View
I have done a lot of PCR to identify a mutation in beta-tubulin. I have two sets of primers, one is for mutant and one is for wild type. I did nearly 100 PCR but I have a problem getting a good...
28 November 2022 5,750 6 View
I'm doing a PCR for detection of Mutation on position 167 in beta tubulin. It is a Nested PCR which a second reaction was performed using the primers Fs + R2 (141 bp) and F1 + Rr (212 bp). The Fs...
19 June 2022 5,688 3 View
Hi everyone I have made a Stock Solution of my primer 100 uM, and I also have a Working Solution of 10 uM. can I convert my working solution 10uM to 5uM directly?! if I want to make a 5uM working...
21 January 2022 5,498 4 View
Hi everyone I tried to do PCR, and I expected to get a band in 121, but is my third PCR that I have gotten 400bp band. I Checked my primer and all of the other parameters. it should be mentioned...
09 December 2021 2,066 10 View
Hello everyone, I have all the hookworm ITS-2 sequences and I want to check where the primers are present and the size of the amplicon with an alignment software (NCBI Blast). what should I do? I...
16 October 2021 1,524 6 View
i want to confirm my finding regarding Ancylostoma Caninum eggs by molecular studies, which is ``ITS-2 amplification``. but the number of eggs in my samples is not to much and i cant get any band...
30 August 2021 936 11 View
