Rowshon Begam

4 Questions 15 Answers 0 Followers

Questions related from Rowshon Begam

Large scale protein purification

I have been trying to purify some proteins to use in a biochemical assay, I need at least 5mg total yield in ~2ug/ul concentration. They all have STREPII tag, I'm using 1ml Strep tactin column in...

04 April 2013 5,260 14 View

How to concentrate protein sample?

I've purified a protein through FPLC, but the concentration is lower than what I need to set up a reaction. Any advice about a good kit to concentrate my protein would be of great help. The...

02 February 2013 4,541 15 View

What would be a good methylating agent for plants?

Does anyone know what I can use in a plant growth medium (MS) as a methylating agent? I see MMS can be used which is of course very toxic; if I must use MMS does anyone know what should be the...

02 February 2013 3,628 11 View

Can you please give us the storage buffer composition of the ScRAD51 used in the strand exchange reaction in this publication?

Our lab is trying to do a strand exchange reaction using ScRAD51 for the first time. We are following the protocol you've explained in this paper. We just wanted to make sure that our ScRAD51...

01 January 2013 204 0 View