Rothangmawi Victoria Hmar

7 Questions 12 Answers 0 Followers

Questions related from Rothangmawi Victoria Hmar

What is the need for spectrum analysis? or what information is obtained?

If a compound shows two peaks in HPLC will it also show two absorption spectrum?

04 June 2019 8,237 5 View

Which data is more important- the activity of enzyme or the specific activity of enzyme?

I have carried out enzyme activity studies for strains A, B , C . Though the activity is different for all, the specific activity is almost same. So which data should I represent or will have more...

17 November 2014 3,122 5 View

During a scale-up studies ,why will the production of a compound reduce?

Studies carried out in shake flask shows a good yield of product, however upon growth in a bioreactor, the yield of the compound decreases. Also the biomass is reduced. The conditions in reactor...

02 November 2014 2,816 12 View

Why do I get a PCR band of the wrong size after cloning, though restriction digestion confirms the presence of the gene?

I cloned a 4.2 kb gene in a vector. Restriction digestion confirmed the presence of the insert. When I carried out PCR with the primers of the gene I get a band but its only 3 kb. How is it...

19 May 2014 9,655 3 View

Why can some DNA fragments/genes only be amplified by high-fidelity polymerases and not by Taq?

Using phusion polymerase I get amplification but when I try using Taq I get no results.

07 May 2014 4,083 4 View

Is a difference in the mRNA level of genes expressed from the same operon possible?

I have cloned 2 genes from a bacterial operon into another bacteria. When i studied the level of mRNA of the two genes, I noticed a difference. The 2nd gene expression was higher than the first....

06 May 2014 2,981 4 View

Will the genes of an operon be expressed differentially?

All genes in an operon are under the control of the same promoter, so will there be difference in the expression of the genes? Are there any reports on this?

01 May 2014 8,346 8 View