Roshini Arivazhagan

3 Questions 5 Answers 0 Followers

Questions related from Roshini Arivazhagan

What is the maximum microRNA concentration that can be used for in-vitro studies?

Hi !! I would like to know what is the maximum microRNA concentration that can be used for in-vitro studies.. Like 100nM or 200nM ect.. Kindly suggest me regarding this. Thanks in advance..

08 May 2015 9,260 2 View

How can I clone multiple fragments into the same vector?

Hi ! Has anyone tried multiple fragment cloning for bigger PCR fragments ? I need to clone a 3kb,3kb and 4kb PCR(total 10kb) fragments into GFP vector. I have difficulties as the fragment size is...

28 November 2014 3,772 10 View

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning?

Hi, I have two enzymes - EcoR1 and Kpn1. Both the enzyme expirations dates in 2010. Can I use these two enzymes for cloning? Will it work and, if yes, will there be proper restriction overhangs?...

24 October 2014 5,609 12 View