6 Questions 4 Answers 0 Followers
Questions related from Rosha P
I'm new to the field of PFGE and have a few questions before I begin the experimental procedures. When I separate out my DNA size of interest, how can I purify it from the gel without using a...
10 October 2013 1,105 6 View
I am doing some Chromatin Immunoprecipitation with histone modifications. I have three biological replicates of my experiment and I run technical replicates when I do qpcrs. Now that I have all...
06 June 2013 761 7 View
My pipettes have somehow been decontaminated with an amplicon and now I get false positives in all of my PCR reactions. Is there a way to decontaminate the pipettes without dismantling them...
04 April 2013 1,108 38 View
The ChIP protocol I'm following has a low salt, high salt, LiCl and 1X TE washes, respectively.The low salt wash buffer has 150mM of NaCl, the high salt wash buffer has 500mM of NaCl and LiCl wash...
09 September 2012 5,063 0 View
Can anyone recommend how to design primers for a ChIP assay that is investigating various histone marks (H3K4 tri methyl, Total H3, H3K9/14 Acetylation, H3K27 tri-methyl, etc)? What gene would be...
08 August 2012 9,331 3 View
This might sound very trivial but what defines a molecule of DNA in cells? I'd think a chromosome is a molecule of DNA because sister chromatids are connected to one another at the centromere/with...
08 August 2012 8,085 12 View
