Rosemary Taylor

3 Questions 3 Answers 0 Followers

Questions related from Rosemary Taylor

Anyone had any issues with eBioscience Foxp3 Transcription Factor Kit?

Hi all, I’ve been trying to use this kit on/off for probably around 8 months, but it hasn’t worked once. I’ve tried optimising the protocol by incubating in fix/perm for longer, incubating in the...

13 February 2018 2,065 16 View

Does DAPI require permeabilisation?

Hi all,I just attempted a DAPI staining and got absolutely no results from the microscope. The obviously answer would be to increase the DAPI concentration, however I was wondering whether DAPI...

25 September 2017 6,877 9 View

Has anyone got a protocol for freezing and thawing naive T cells?

My supervisor has asked me to find a protocol for freezing and thawing naive T cells, with details regarding the % recovery of the T cells once thawed, but I'm struggling to find any information...

11 May 2017 3,261 3 View