Rosa Mistica Coles Ignacio

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Questions related from Rosa Mistica Coles Ignacio

Wrong size of inserts and multiple bands after restriction enzymes digest of plasmid?

Band 2 and 3 (after the marker) have wrong insert size, the size should be 900bp but it showing here 2000bp. The pcr product I begin with has 900bp as confirmed by gel electrophoresis. What are...

04 April 2018 8,420 2 View

Hi everyone! Does anyone here have an exeperience on transfecting DOV13 ovarian cancer cell line?

I want to check the TLR4-mediated activation of NF-kB in response to LPS. So, I transfected my DOV13 cells with CCL20 for 24h. I did this in 24-well plate, however I had problem with the cells,...

02 February 2017 6,734 0 View

Hi! Is anyone here has an experience doing RNA sequencing of mouse pre-adipocyte (3T3-L1) and the differentiated 3T3-L1?

Can you please give me an overview? I'm interested in doing gene expression profiling of the said cell lines and further narrow down our target gene for study.

10 October 2016 5,128 0 View