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Band 2 and 3 (after the marker) have wrong insert size, the size should be 900bp but it showing here 2000bp. The pcr product I begin with has 900bp as confirmed by gel electrophoresis. What are...
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I want to check the TLR4-mediated activation of NF-kB in response to LPS. So, I transfected my DOV13 cells with CCL20 for 24h. I did this in 24-well plate, however I had problem with the cells,...
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Can you please give me an overview? I'm interested in doing gene expression profiling of the said cell lines and further narrow down our target gene for study.
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