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Questions related from Rosa Aledo
I would like to transitory transfect ASC52telo to introduce plasmids for GFP-crispr editing, I tried several liposome derived samples but the efficiency is very bad, does anybody expericence with...
27 August 2021 8,324 3 View
I have several cells transfected with a differents gene deletions fused to GFP and in order to make a qPCR to detect all the constructs, not only for the specific gene, I tried a commercial GFP...
30 November 2020 710 1 View
I have cloned a fragment of a human membrane protein fused with GFP in a lentiviral vector, once virus were produced in HEK293 I infected human endothelial cells, my control (only GFP lentivirus)...
12 March 2020 8,346 3 View
I used genomatyx to analyze transcription factors in promoters sequences, but now I have many problems to login, so does anyone knows another facility to do this?
14 October 2016 7,464 4 View
I treat macrophages cultures with LPS and I look in supernatant of cell culture the secretion of several proteins. I used all the supernatant for each well to be concentrated at equal volume and I...
03 November 2015 7,383 27 View
I would like to use microsatellites in TCF7L2 human gene , I need to know localization, heterozigosity and frequency in population. What data base can bring me all of the specifications?
28 October 2015 1,323 3 View
I express a recombinant proteins in E. coli (wild type and mutant), the mutant one is much less expressed than the wild type and the culture is slower (time to attain DO 600 of 0.6 before to add...
14 October 2015 8,298 6 View
I expressed a recombinant protein in a procariotic system but I need to eliminate endotoxins, LPS, I tried using polyLysin resin but it doesn't work (I loose too much protein). I read that using...
15 April 2015 2,934 14 View
Sometimes it is not possible to see single bands in EMSA, and complexes remain as a big spot not resolved. Could anyone explain to me how to find a solution?
30 October 2013 9,337 4 View
I made cDNA constructions to produce proteins with GFP or dsRED (clontech), and one construction with dsRED after transfection in HeLa cells. I obtained RNA by qPCR, red cells by confocal...
24 January 2013 8,502 13 View
