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I would like to transitory transfect ASC52telo to introduce plasmids for GFP-crispr editing, I tried several liposome derived samples but the efficiency is very bad, does anybody expericence with...
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I have several cells transfected with a differents gene deletions fused to GFP and in order to make a qPCR to detect all the constructs, not only for the specific gene, I tried a commercial GFP...
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I have cloned a fragment of a human membrane protein fused with GFP in a lentiviral vector, once virus were produced in HEK293 I infected human endothelial cells, my control (only GFP lentivirus)...
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I used genomatyx to analyze transcription factors in promoters sequences, but now I have many problems to login, so does anyone knows another facility to do this?
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I treat macrophages cultures with LPS and I look in supernatant of cell culture the secretion of several proteins. I used all the supernatant for each well to be concentrated at equal volume and I...
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I express a recombinant proteins in E. coli (wild type and mutant), the mutant one is much less expressed than the wild type and the culture is slower (time to attain DO 600 of 0.6 before to add...
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I expressed a recombinant protein in a procariotic system but I need to eliminate endotoxins, LPS, I tried using polyLysin resin but it doesn't work (I loose too much protein). I read that using...
15 April 2015 2,909 14 View
Sometimes it is not possible to see single bands in EMSA, and complexes remain as a big spot not resolved. Could anyone explain to me how to find a solution?
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I made cDNA constructions to produce proteins with GFP or dsRED (clontech), and one construction with dsRED after transfection in HeLa cells. I obtained RNA by qPCR, red cells by confocal...
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