Ronny Khalil

4 Questions 5 Answers 0 Followers

Questions related from Ronny Khalil

How can i regulate my liquid chromatography flow rate?

I am trying to isolate lipoprotein A1 by running liquid chromatography on Sephacryl S200 packed column, 5x150cm. The buffer (moving phase) goes thru the top via gravity, and on the bottom i have a...

02 February 2016 5,437 3 View

Does anyone have a protocol for the Homocitrulline assay?

I am making carbamylated LDL, and need to run the homocitrulline assay to verify that the end product works. Could anyone kindly provide me with a protocol to the homocit assay for this purpose Thanks

01 January 2016 8,657 2 View

How do I regulate the flow in an ion exchange column?

I am trying to isolate a nerve growth factor, and the protocol calls for various flow rates throughout the process. I have a really old flow adapter in the column, but I really cant seem to...

11 November 2015 8,305 6 View

What is/are the likely reason(s) for the separation of the fluorescent DiI and LDL?

Hello everyone, I made low density lipoprotein (LDL) labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) and prepared for macrophage like cells (P388D) for...

12 December 2014 5,669 1 View