Ronni Cohen

2 Questions 5 Answers 0 Followers

Questions related from Ronni Cohen

Why does a Gibson Assembly produce DNA that cannot be digested?

Hi everyone, I've used Gibson assembly a couple of times in the past and it mostly went smoothly. Now I'm having strange results. First- the technical stuff: I'm assembling a 3 segments plasmid,...

01 January 2018 3,170 3 View

Why might I be getting inconsistent Cre-recombination in NestinCreER and Gli1CreER mice after Tamoxifen injections?

Hello everyone, I've been struggling for a while with two Tamoxifen dependent Cre expressing mice lines. I breed both strains with the reporter line Rosa26. Have done extensive genotyping to...

12 December 2017 4,045 2 View