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Questions related from Rongqi Jia
I am now preparing a DNA chain with sticky ends, one side of which is a 4nt sticky end and the other side is a 35nt sticky end. It is prepared by primer return and is divided into four groups:...
17 June 2025 2,882 3 View
I need to make a DNA strand consisting of two 35 nt sticky ends and 147 bp of double-stranded nucleic acid.I achieved this with primers that added spacer. However, there are still a large number...
24 February 2025 7,867 2 View
I designed and specifically cross-linked a protein complex. How can I determine the stability of this complex after cross-linking? My experimental environment is quite rudimentary, and I don't...
04 November 2024 5,682 2 View
This ssDNA is composed of a 160bp double-stranded region in the middle, with 35nt single-stranded oligonucleotides at each end. Due to budget constraints, I don't have the opportunity to order two...
04 September 2024 2,695 1 View
I am currently constructing a variant of a protein, where I mutated threonine to cysteine. However, when I followed the previous expression steps, the expression level was very low. What could be...
13 August 2024 8,783 4 View
I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...
17 July 2024 2,824 2 View