Rongqi Jia

6 Questions 1 Answers 0 Followers

Questions related from Rongqi Jia

Why do DNAs of the same length have different positions in electrophoresis?

I am now preparing a DNA chain with sticky ends, one side of which is a 4nt sticky end and the other side is a 35nt sticky end. It is prepared by primer return and is divided into four groups:...

17 June 2025 2,895 3 View

I wanted to find a way to get pure DNA，Can you give me some pointers?

I need to make a DNA strand consisting of two 35 nt sticky ends and 147 bp of double-stranded nucleic acid.I achieved this with primers that added spacer. However, there are still a large number...

24 February 2025 7,874 2 View

How can I determine the stability of protein complexes under rudimentary conditions?

I designed and specifically cross-linked a protein complex. How can I determine the stability of this complex after cross-linking? My experimental environment is quite rudimentary, and I don't...

04 November 2024 5,697 2 View

I need an unusual ssDNA. How can I obtain it?

This ssDNA is composed of a 160bp double-stranded region in the middle, with 35nt single-stranded oligonucleotides at each end. Due to budget constraints, I don't have the opportunity to order two...

04 September 2024 2,703 1 View

Does a point mutation affect protein expression?

I am currently constructing a variant of a protein, where I mutated threonine to cysteine. However, when I followed the previous expression steps, the expression level was very low. What could be...

13 August 2024 8,789 4 View

How can I prevent protein subunits within a protein complex from dissociating without altering the overall structure?

I have a protein complex in which some dimers easily dissociate. What methods can I use to stabilize these dimers within the complex without altering the overall structure of the complex? Will...

17 July 2024 2,840 2 View