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We are using pMSCV for transit expression of a protein.: 1. The gene was cloned in (between XhoI/EcoRI). 2. Transfection with lipofectmin 3000 to 293t cell. 3. After 48 hrs, GFP can be...
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I'm new in NGS data analysis. Recently, we sequenced a hairpin library (~1000 shRNA) for quality and We already got the fastq file from illumna. We need to get information from this fastq file:...
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