Why is there no yellow formation? (Alpha Glucosidase inhibtion assay)?

I'm currently doing the assay for inhibition of glucosidase. 50 uL of extract and 50 uL of glucosidase were incubated for 15 mins at 37 C. Then, 50 uL of 4-nitrophenyl-a-D-glucopyranoside. It...

10 September 2019 1,275 3 View