UHPLC instrument issues with NH4PF6 and KPF6 (100 mM, pH 3.5) as buffer salts/additive in the mobile phase?

I am developing a UHPLC method for content and purity for a peptide based on 100mM KPF6 and 100mM NH4PF6 pH 3.5. TFA as an ion pair reagent does not give the required separation performance. My...

21 April 2023 9,371 4 View