Problems making Lentivirus with pLKO-Tet-On plasmid - can anyone help?

We have made a lot of pLKO.1 based lentivirus with no problem. I am currently trying to make the pLKO-tet-on based lentivirus using pSPAX and pMD2 vectors for packaging in HEK293TLA cells and we...

27 September 2013 9,239 1 View