Rojin Falah

4 Questions 4 Answers 0 Followers

Questions related from Rojin Falah

Why PC12 cells do neurite outgrowth when expose to NGF but western blot does not show TrkA overexpression in pretreated PC12 cells compare to WT PC12?

I have been treated PC12 cells with 100ng/ml NGF for 10 days, and lysed them using RIPA buffer for western blot. I got no bands for primed cells, but for wild type cells. Does anyone know why?!

25 July 2023 2,516 3 View

Is there any optimized HPLC condition for Nerve Growth Factor (NGF)?

I am trying to get a good HPLC peak for NGF, but I could get 2 or 3 peaks in row, or sometimes a very wide peak.

17 June 2022 349 0 View

Is precoated plate with collagen required for PC12 cells to do the neurite outgrowth?

I am doing PC12 cells neurite outgrowth in non-coated plate. Based on the ATCC product information sheet, the cells are normally floating clusters and few scattered lightly attached cells. I can...

09 May 2022 8,382 5 View

Do priming PC12 cells with NGF upregulate TrkA expression level?

I plated PC12 cells in 6 well plate, and now I am trying to prime the cells with 50ng/ml of NGF. After incubation for 11 days, do the cells express more TrkA compared to the unprimed ones?

22 April 2022 787 1 View