Roisin Moloney

4 Questions 1 Answers 0 Followers

Questions related from Roisin Moloney

How can you get OPCs to become myelinating oligodendrocytes?

Hi all, I'm currently trying to isolate oligodendrocytes from GA62 (term is GA70) guinea pigs and am having a bit of trouble getting them to survive. I take the tissue and digest with papain and...

16 April 2021 7,378 1 View

Is it possible to measure dopamine receptors (through RT-PCR or ICC) in primary culture?

I would like to examine effects of particular drugs on D1 and D2 receptors using culture, but wondered if this is possible as people tend to look more at TH-positive cells. Thanks!

16 March 2021 8,980 3 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

15 February 2021 3,862 4 View

Accutase didn't detach oligodendrocytes from lysine coated flasks, has anyone else had this problem?

I've coated T75 flasks in 0.1mg/mL poly-D-lysine but when I went to use accutase to detach them they wouldn't budge. Tried putting them in the incubator for a couple of hours and nothing, wondered...

18 January 2021 4,291 1 View