Rohulla Vaseq

4 Questions 2 Answers 0 Followers

Questions related from Rohulla Vaseq

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

05 August 2024 9,869 7 View

How to gate precisely the live cells stained with fixable viability stains for flow cytometery?

I have a question maybe looks naive. I want to stain my cells with fixable viability stains. If we look at the chemistry of these stains, even live cells, although dim, can be stained. For...

05 June 2023 9,153 2 View

Must (or Can) we use the annexin buffer in antibody staining after annexin staining?

What if we use annexin buffer for antibody staining? Let me ask in another word. If we want to stain in two separate steps, step one annexin and step two antibody staining, must we use annexin...

25 January 2023 7,485 4 View

How can I coat poly lactic glycolic acid (PLGA) on the well for ELISA assay?

Hi there well as you read the question, I want to coat PLGA on the polystyrene plate for antibody detect with ELISA assay. Is it possible to use usually way for peptid coating? because PLGA is not...

06 May 2016 650 1 View