Rohit Kunar

5 Questions 14 Answers 0 Followers

Questions related from Rohit Kunar

In silico softwares for Prediction of Protein-RNA binding?

I am working on an RNA binding protein, against which I do not have an antibody. Neither is the crystal structure known in the particular organism. I am interested to have an in silico prediction...

25 June 2018 6,075 2 View

Can anyone help me with the Optimisation of Induction for a GST fusion protein?

I have a clone of my protein coding ORF in pGEX-4T-1 vector which should produce a fusion protein of ~115kDa. Snapgene Analysis of the clone (sequence) shows that the clone is in proper...

05 February 2018 2,365 6 View

How can I identify enhancer-promoter communication at DNA level (possibly visually) in drosophila?

I have a mutant Drosophila wherein I suspect that a certain gene has insulator sequences (resembling LTRs) inserted in the region coding for 5'UTR. I wish to search, detect and identify the...

04 May 2015 9,367 2 View

Do you have any reports on methylation in tumorigenesis in drosophila?

Can anyone provide me with any information on CpG or CpA or CpT methylation in Drosophila, and whether that ihas any role in the larval states?

04 May 2015 5,257 6 View

How can I clone a PCR generated 9 kb amplicon in a 7.8kb vector?

I am trying to clone a full length gene with flanking sequences (a total size of 9 kb) in the Drosophila expression vector pCaSpeR4. I had constructed primers with sites for BamHI and KpnI in the...

18 December 2014 5,215 12 View