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Questions related from Rohit Kunar
What are the online MD simulation servers available for assessing models generated by Alphafold? My protein is 680 residues long. I was trying to model it in ITASSER and Alphafold. The model...
14 March 2025 8,779 2 View
I am working on an RNA binding protein, against which I do not have an antibody. Neither is the crystal structure known in the particular organism. I am interested to have an in silico prediction...
25 June 2018 6,128 2 View
I have a clone of my protein coding ORF in pGEX-4T-1 vector which should produce a fusion protein of ~115kDa. Snapgene Analysis of the clone (sequence) shows that the clone is in proper...
05 February 2018 2,413 6 View
I have a mutant Drosophila wherein I suspect that a certain gene has insulator sequences (resembling LTRs) inserted in the region coding for 5'UTR. I wish to search, detect and identify the...
04 May 2015 9,412 2 View
Can anyone provide me with any information on CpG or CpA or CpT methylation in Drosophila, and whether that ihas any role in the larval states?
04 May 2015 5,300 6 View
I have a linear PCR Amplicon of 8715 bp. It has BamHI sites at its ends with 5' flankers of 15 bp at each end. I need to digest it and clone it. However, I have a feeling that it is not being...
19 February 2015 1,849 6 View
I have been trying to clone a PCR amplified full length gene from Drosophila melanogaster in the BamHI-KpnI sites of pCaSpeR4, but my strategies fail every time. The amplicon is 8.6 kb in length....
09 January 2015 8,617 5 View
I am trying to clone a full length gene with flanking sequences (a total size of 9 kb) in the Drosophila expression vector pCaSpeR4. I had constructed primers with sites for BamHI and KpnI in the...
18 December 2014 5,285 12 View
I am working on a gene whose enhancers I would like to know. Instead of the conventional P element based enhancer trap, is there any prediction tool which can predict the enhancer of the given...
18 December 2014 7,974 4 View
