Rohit James

4 Questions 13 Answers 0 Followers

Questions related from Rohit James

When designing constructs for Bacillus subtilis spore surface display do you include the START codon of the passenger protein?

In B.subtilis spore surface display cot partner with native promoter is fused in frame with a passenger protein via a linker like GGGGS. The assumption here is that a fusion mRNA is formed, and...

01 March 2023 2,854 1 View

Can PCR product be used as a template for reamplification? - Practical tips?

The future expected problem - So, I plan to join two DNA fragments A and B by overlap PCR. Amplify individual fragments use them together as template and then amplify the combined fragment ab...

13 April 2021 4,453 16 View

What filter can I use to take gel images with a smartphone?

So we use Ethidium bromide to stain our agarose gels for dna extraction and analysis. The software in our gel doc is malfunctioning so we are unable to capture images. So now we are using our...

27 November 2018 2,959 7 View

Any concerns regarding amplification of gene of interest from genomic DNA of Bacillus subtilis with end modified primers?

So, I would like to amplify a gene of interest (goi) from Bacillus subtilis genomic DNA. I have designed primers with restriction sites, a linker in the Forward primer and a His tag in...

20 November 2018 9,856 8 View