Rohini Sachdeva

3 Questions 10 Answers 0 Followers

Questions related from Rohini Sachdeva

How do I standardize elution conditions of an insoluble protein?

I am working on a truncated viral protein. I have expressed my protein in pET15b system, most of the protein is insoluble thats is in the inclusion bodies. I used 8M urea to dissolve the pellet...

05 May 2015 3,870 4 View

Problem Cleaving the tag off of GST tagged protein using Sigma thrombin clean cleave kit, that has bovine thrombin bound on resin. What can I do?

I expressed my target protein in pGEXT-4T-2 vector. I got fairly nice amount of protein after purification using GST sepaharose 4B beads. Now when I tried cleaving off thrombin using on-column...

09 September 2014 5,244 3 View

Taqman qPCR for absolute quantification of viral RNA copies?

Hi, I am using a taqman qPCR for absolute quantification of viral RNA copies in the susceptible cells at different time points after infection with virus. The way I do that is to do a standard...

07 July 2014 8,376 4 View