Rohini Mehta

2 Questions 4 Answers 0 Followers

Questions related from Rohini Mehta

Does sloughing off of cells in patches during staining indicate insufficient cell fixing?

Hepatocytes were fixed in 10% NBF (Sigma) for 10 min. While washing and staining there were regions/patches where cells were washed away, something like bald spots (I was staining by oil red o and...

10 October 2012 6,388 10 View

I am curious on what is the purpose of using 60% isopropanol after fixing cells with 10% neutral buffered formalin?

I am staining for lipids in adherent cells using oil red O. I often see this step in various staining protocols for adherent cells.

09 September 2012 5,272 10 View