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I transfected SARS-CoV-2 Spike (https://www.addgene.org/141382/) in HEk293T cells and 48 h later collected cell lysate in Pierce lysis buffer containing protease inhibitor cocktail. However, upon...
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My VeroE6 cells show this strange clumps in the monolayer after about 3-4 passages post thawing (see attached pic). Once this phenotype appears, the cells don't get infected and become worse over...
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