Rohail Jawed

6 Questions 17 Answers 0 Followers

Questions related from Rohail Jawed

How to validate Indirect ELISA without standards?

I am working with the antigen on Indirect ELISA.I have really confused results, few samples are true positive and few samples are true negative but many samples are in between negative and true...

01 January 2016 574 7 View

Why is my protein disappearing from the SDS PAGE gel?

I am working with Recombinant His tag proteins with molecular size of 10 and 5 kDa, after the completion of gel run, i stained with coomasive blue dye .After few minutes of destaining i can see my...

07 July 2015 6,104 4 View

How do I optimize my non-denaturing PAGE for my protein with acidic PI?

I tried several different conditions but my proteins is not running on gel.I want to optimize non denaturing PAGE,PI of my protein is Acidic,please suggest me the best protocol and condition.Thanks

03 March 2015 3,117 4 View

I am working on optimization of native PAGE,I am unable to get my protein of interest after staining,what could be the reason?

I am working on optimization of native PAGE but I am unable to get my protein of interest after staining. I have read several protocols but I am still confused. I am also worried about the loading...

02 February 2015 3,785 23 View

I am unable to get the band on Western blotting but the protein is highly positive on ELISA, what could be the reason?

I am working with 4 pure mitochondrial enzymes without any tag protein whenever I used this enzyme on ELISA I got the highly positive signals but when I do western blotting only 2 proteins give...

01 January 2015 4,465 16 View

Does anyone have an idea as to why I am unable to get the correct size of human protein cloned in a pET28a vector?

I have cloned a human gene into the pET28a His tag vector but I am unable get the correct size of my protein. I have confirmed recombinance by sequencing and restriction digestion.The correct size...

06 June 2014 3,592 15 View