Rockson Anokye

2 Questions 1 Answers 0 Followers

Questions related from Rockson Anokye

How do viable mice sperm look like after freeze and thaw?

I used R18S3+MTG cryopreservation media to store sperms, obtained from the cauda epididymis of male mice. After one week, I thawed the sperm from liquid nitrogen, using a 37 C water bath. I...

29 May 2025 2,103 1 View

How do we maintain single cells viability after fluorescent activated cell sorting during CRISPR/Cas9 knockout experiment?

I am new to CRISPR/Cas9 genome editing. Currently, I am carrying out CRISPR/Cas9 knockout of a gene and as part of the process, I carried out electroporation of RBL-2H3 cells with PX458 + gRNA...

20 May 2025 3,316 1 View