Rocco Zerlotti

2 Questions 1 Answers 0 Followers

Questions related from Rocco Zerlotti

French press on mammalian cells to obtain ISO vesicles?

Hello, I´m trying to obtain ISO (inside-out) membrane vesicles from CHO cells using a French press. I already tried once - with no results - on 1g CHO cells, lysis buffer composed by 50 mM KCl...

02 August 2021 9,381 0 View

What is the best way to avoid as much damage as possible upon refreezing cells?

Hello, I have a ~2 g frozen pellet of CHO cells overexpressing a membrane protein (stored at -80°C) which will be used for membrane vesicles preparation. I need to resuspend the pellet, split it...

05 July 2021 2,066 5 View