Does someone have experience with blocking calcium channels on primary cortical culture by means of Cadmium/Nickel/Cobalt-Chloride?

I would like to perform some extracellular recordings on MEAs from primary cortical neurons, where I block Ca+ channels by using CdCl, NiCl, and CoCl. My doubts are about proper concentrations and...

20 April 2015 4,602 4 View