Robson Tramontina

2 Questions 2 Answers 0 Followers

Questions related from Robson Tramontina

What is the simplest method to recover enzymes from inclusion bodies in recombinant E. coli?

I've tried 4 strains: Arctic Express, Rosetta, Origami, and BL21. None of them could make my enzymes soluble. I did enzymatic experiments on the pellet lysate (where the inclusion bodies are...

08 August 2015 2,674 3 View

Does E. coli strain K011 support pet28A vector to express intracellular proteins?

I transformed K011 with the vector pet28A containing an enzyme. That enzyme was expressed successfully in BL21 (de3) strains. Moreover, PCR analysis showed that the transformation worked....

01 January 1970 9,156 6 View