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Questions related from Robinson White
I am working with HL-60 (suspension cell) and HuH7 (adherent cell). Normally it is seen that the cells become confluent within 3 days after subculture; while sometimes it does not. It may take 1...
02 July 2017 5,589 3 View
I would like to differentiate THP-1 cell to macrophage using PMA and transfect pAcGFP vector with Lipofectamine ltx transfection reagent (as it is available). Is there any standard protocol of...
15 June 2017 4,256 2 View
I have got a sequence of my human promoter of interest-reporter (pGL4) vector of 3Kbp. The promoter sequence is in capital letters (representing the nucleotides) and the reporter vector sequence...
29 March 2017 4,634 4 View
I performed a dual luciferase assay using pGL3 firefly luc as experimental reporter and pRL-TK renilla luc as internal control (Promega assay kit). I used a manual luminometer to analyze my...
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I want to perform the dual luc. assay using Hl-60 cell line which is a floating cell. Therefore, I explored different papers but no brief protocol found about the assay using this floating...
15 March 2017 8,992 1 View
I run 0.7% agarose gel electrophoresis of my plasmid DNA sample consisting of GFP vector using NheI and HindIII restriction enzyme. I used two types of plasmids, i.e., isolated by mini prep-alkali...
06 January 2017 7,413 1 View
I need to transfect GFP vector and Luciferase vector plasmid DNA into the HL-60 floating cells. I will have to use X-tremeGENE HP DNA Transfection reagent from ROCHE. If anyone already experienced...
24 November 2016 5,589 2 View
Generally, we know that ERK signaling is responsible for controlling cell growth, proliferation, differentiation, and apoptosis. However, I found this signal important for cytokine expression in...
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