Robin Moll

7 Questions 16 Answers 0 Followers

Questions related from Robin Moll

Is there a reduction in the sensitivity of PCR when using universal primers with degenerate sequences?

I am using a pair of primers each oligo has a degeneracy of 4, so an overall degeneracy of 8 when they are used together. The primers are for the amplification of 16S rDNA so are being used to...

05 May 2015 4,621 3 View

Can anyone help me with statistical analysis of qPCR data?

I am just wondering if qPCR data should normally be analysed with a two-tailed test because we are wanting to test the sig of expression in either direction (up- or down-reg). But we can see the...

09 September 2014 1,403 3 View

Is it strictly necessary to differentiate THP-1 cells to a macrophage phenotype before using them in a model of infection?

I'm just curious to know if anyone has ever infected THP-1 cells with whole bacteria without differentiation? I know that monocyte THP-1 cells are very sensitive to LPS and so, if measuring gene...

05 May 2014 6,874 2 View

What values do we use for statistical testing of qPCR delta delta ct (ddCt)?

I have performed ddct with mean ct values for 15 test replicates and mean ct values for 11 calibrator samples. I have all my values for ddct so it's not calculating the my expression fold change...

04 April 2014 2,962 10 View

Without immunological markers, what cells can be accurately identified in a simple H&E section?

In the literature, researchers often use antibodies against immune cell markers (CD4, CD11 and CD68) to quantify particular cells occurrence. What cells can be identified without such...

04 April 2014 1,537 38 View

Should experimental triplicates have very similar Ct values?

I have performed an experiment in which THP-1 cells were exposed to a bacterial strain (heat killed) as a model of infection. I decided upon an MOI of 100 and performed the experiment with three...

01 January 2014 1,438 13 View

THP-1 Cells acting strange after freezing down and reviving?

I would like some advice regarding THP-1 cells after freezing stock vials. I was growing these cells perfectly; they were behaving wonderfully to the letter. I was using the ATCC recommended...

05 May 2013 5,916 7 View