Robin Sachdeva

4 Questions 6 Answers 0 Followers

Questions related from Robin Sachdeva

Why my western blot (Ponceau stain) has straight vertical lines?

I loaded 40ug of protein for each sample. During 15 min of PAGE run, i saw a ''mountain like structures'' only in the wells where i saw vertical straight lines in Ponceau staining. Some more...

13 November 2017 1,754 8 View

Does anyone saw decrease in baseline calcium levels upon agonist addition in FURA 2 AM calcium imaging?

I am using MCEC (Mouse cardiac Endothelial cell line) and using Methylglyoxal as agonist. I would like to know what could be possible ways to explain this phenomenon. I have also tested and it is...

04 August 2016 6,989 4 View

Why i saw absorbance in MTT assay where there is no living cells (checked through microscope)?

I am wondering why i am seeing the absorbance (from pink color) at the last step of MTT assay in the wells where there are no living cells (checked with microscope). Colour of MTT reagent looks...

21 June 2016 3,939 3 View

Where do the enzymes for Glycolysis in denucleated mature RBCs come from?

Mature mammalian RBCs are denucleated. It means that RBCs cannot code for any protein. We also know that Glycolysis occurs in RBCs but from where RBCs did they get all the enzymes needed for...

10 November 2014 5,972 6 View