Robin J Horst

2 Questions 8 Answers 0 Followers

Questions related from Robin J Horst

Which positive and negative controls for H3K4me3 ChIP in Arabidopsis would you recommend?

Can anyone recommend good controls for H3K4me3 ChIP that I can use for a qPCR assay? I'm looking for a region that generally has this mark (positive control) and one that generally does not have...

07 February 2014 4,326 3 View

Cross-linking for ChIP in plants: Fixation before or after grinding?

I'm doing ChIP on a TF that is expressed in cell types with low density and am struggling to get reproducible results. My protocol works fine for other TFs. I crosslink my material in 1%...

21 February 2013 9,120 2 View