Robin Crucitti-Thoo

2 Questions 3 Answers 0 Followers

Questions related from Robin Crucitti-Thoo

Is it necessary to perform both forward and reverse Sanger sequencing to verify the presence of a gene in a sample?

Hello :) I am looking at the presence of toxin biosythensis genes in lake sediments. Nothing fancy, since I only want to verify the specificity of PCR results for primers related to marker genes...

28 May 2024 350 4 View

Is it common practice to verify PCR results with Sanger prior to NGS?

Hello, Since a large part of my budget will go on NGS and I don't have the money to repeat it, I want to make sure that it will work. The PCR products look good on the gel (nice bands of the...

22 April 2024 7,748 3 View