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Questions related from Robert Allan Marmion
I would like to assemble a linear fragment together in Gibson and PCR amplify the assembled fragment for cloning. Has anyone tried this? It shouldn't work because the exonuclease will destroy the...
03 March 2015 9,379 13 View
The Gibson Assembly Mix is assembled on ice for obvious reasons, including not chewing back those ends too far. However, is there a suggestion to go into a machine pre-warmed to 50 degrees C or...
03 March 2015 8,257 1 View
