Robert H Chow

4 Questions 8 Answers 0 Followers

Questions related from Robert H Chow

Can anyone suggest a fluorescent dye that can be loaded into neurons during patch clamp, and that will stay in the neurons after fixing the tissue?

We want to perform image analysis to characterize neuron branching, post patch clamp experiment, in fixed neurons.

11 November 2017 820 10 View

Does anyone have experience with the Oxford Nanopore Technologies MinION?

We are working on single-cell RNAseq, and I have been wondering whether the MinION can process RNA from single cells.

10 October 2016 9,738 2 View

Has anyone observed differences in apparent electrophoresis gel locations of DNA bands with Sybrsafe compared with ethidium bromide?

We prepared PCR products and ran a gel on a new SynGene gel doc system with Sybrsafe dye and compared the result with a gel with ethidium bromide. In one case the bands were located at ~5.5kb...

02 February 2016 8,675 10 View

Can anyone suggest a cell line that reliably fires fast action potentials?

We are testing various drugs that may alter the firing rate, and currently we use primary cultured neurons and neuroendocrine cells, which is a lot of work -- that's why we were wondering whether...

12 December 2013 9,229 5 View