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Questions related from Robert Jackson Mobley
When performing ChIP, I use a sonics vibra-cell sonicator to sonicate my samples. Then I spin the samples at 13k rpm for 10' and transfer the sup to a new tube. I run 5ul of my sonicated sample on...
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In our hands Drosophila Spike-in often fails to generate enough reads for a statistically relevant normalization and it is pretty expensive. This computational spike-in free method has recently...
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