Robert Maughan

2 Questions 4 Answers 0 Followers

Questions related from Robert Maughan

What is the lowest acceptable CT/CP in a QPCR experiment?

I have read and heard quite a bit that the lowest admissable CT/CP value is ~12-15. My idea for the basic logic of this is that the reaction is likely to have been overloaded with initial...

21 March 2014 9,449 6 View

Can primary pre-adipocytes be frozen and preserved for future culturing?

I am currently working on a protocol for the isolation and culturing of human pre-adipocytes taken from sub-cutaneous fat biopsies. I am wondering if it is advisable to freeze the isolated...

02 August 2012 4,101 8 View