Is it possible to add an additional coat of gelatin on poly-L-lysine coated flasks for cell culture and how will it affect the cultured cells?

We are planning to culture HUVECs. However, we have a large stock of poly-L-lysine coated flasks and everyone is suggesting to culture the cells on either fibronectin or gelatin coated flasks or...

10 October 2013 3,077 9 View