On an average how much time it takes for Fluo-4 dye signal to achieve an equilibriated stage in hippocampal neuron?

Flu-4 is a widely used calcium sensing dye (200+ publication in pubmed; where it has been used in neurons). I wonder in how much time this dye will reach a saturation point (stable signal) ? Is...

01 December 2016 4,809 5 View