Rm Mal

2 Questions 7 Answers 0 Followers

Questions related from Rm Mal

Is there a way to normalize CD spectra of identical proteins based on absorbance?

I have two identical proteins that were diluted from a stock but run on different days. There are slight differences in the spectra due to pipetting error and concentration differences because the...

11 November 2016 6,048 8 View

Does anyone know how long a disulfide bond in a protein remains reduced?

I am looking to reduce my protein and then remove the DTT quickly using a desalting spin column for subsequent reactions. Does anyone know how long the disulfide bond will remain reduced?

05 May 2016 9,104 11 View