Rm Mal

2 Questions 5 Answers 0 Followers

Questions related from Rm Mal

Does anyone know how long a disulfide bond in a protein remains reduced?

I am looking to reduce my protein and then remove the DTT quickly using a desalting spin column for subsequent reactions. Does anyone know how long the disulfide bond will remain reduced?

16 May 2016 5,453 10 View

Steady state Kd affinity and threshold in surface plasmon resonance (SPR)

I would like to know if there is a quantitative justification for using a specific threshold to calculate steady state Kd affinity using surface plasmon resonance (SPR). Some people say 10% but I...

01 January 1970 2,267 3 View