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Questions related from Rizki Amelia
Do you have any suggestion how to get rid these non specific bands? These are my protocols: 1. Osteoblast cells were cultured in 100-mm cell culture dishes in serum-deprived α-MEM overnight. 2....
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I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...
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