Riya Karan

3 Questions 11 Answers 0 Followers

Questions related from Riya Karan

Protein(beta-lactamase) is overexpressed but why the yield during purification through NI-NTA affinity chromatography is not satisfactory ?

As I have attached the over-expression profile of protein betalactamase I am working on, it seems that the over-expression is satisfactory. But through Ni-NTA chromatography the purification is...

03 August 2024 8,570 6 View

Protein(beta-lactamase) is well expressed but why E.coli cells are not responding in MIC experiment?

Currently I am working on beta-lactamase enzyme. Proteins are well expressed and purified (plasmid pET28a)but not getting result of MIC in BL21/Top10 E.coli host cells while treating with...

14 June 2023 9,013 4 View

Why filamentous/thread like bacterial growth is appearing in secondary culture(E. coli BL21) while primary culture looks fine in protein purification.

Currently I am working on beta-lactamases. I am using pET-28a vector(present kanamycin resistance gene) for cloning purpose, and transformed in E.coli BL21(DE3) bacterial cells. During...

01 January 1970 128 9 View